The Laboratory’s main field of interest covers the structures of the so-called cap, i.e. the 5’ end of mRNAs and many snRNAs. Cap analogs synthesized in the past by us, including the natural trimethylguanosine cap and cap-4, significantly contributed to the discovery of the biological role of these structures.
Currently our activity is focused on interdisciplinary studies on the molecular mechanisms of interactions between cap structures and protein regulator factors that are involved in processes of protein biosynthesis and intracellular mRNA transport.
Within a wider scope mRNA metabolism the research on biosynthesis and degradation of cap structures by capping and decapping enzymes is currently being carried on. Attempts are made to combine basic research with practical applications such as by designing and synthesis of mRNA transcripts stable and translationally highly active in a cell, that can be used for biotechnological protein production and development of anti-cancer vaccines. In our research we use a broad range of chemical, biological and biophysical methodologies. Our team cooperates with several specialist laboratories located both in Poland and abroad.
Group Leader:
Prof. Edward Darżynkiewicz
Senior Scientist:
Renata Grzela, PhD
Postdoctoral Fellow:
Magdalena Chrabąszczewska, PhD
PhD student:
Michał Tyras, MSc
Research Technicians:
Elżbieta Bojarska, PhD
Aleksandra Ferenc-Mrozek, MSc
Michał Magda, MSc
Beata Miedziak, MSc
Prof. Edward Darżynkiewicz
Senior Scientist:
Renata Grzela, PhD
Postdoctoral Fellow:
Magdalena Chrabąszczewska, PhD
PhD student:
Michał Tyras, MSc
Research Technicians:
Elżbieta Bojarska, PhD
Aleksandra Ferenc-Mrozek, MSc
Michał Magda, MSc
Beata Miedziak, MSc
Peptide deformylases from Vibrio parahaemolyticus phage and bacteria display similar deformylase activity and inhibitor binding clefts
Grzela, R., Nusbaum, J., Fieulaine, S., Lavecchia, F., Desmadril, M., Nhiri, N., ... & Giglione, C. (2018).
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1866(2), 348-355.
Grzela, R., Nusbaum, J., Fieulaine, S., Lavecchia, F., Desmadril, M., Nhiri, N., ... & Giglione, C. (2018).
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1866(2), 348-355.
Hydrolytic activity of human Nudt16 enzyme towards dinucleotide cap analogs and short capped oligonucleotides
Grzela R, Nasilowska K, Lukaszewicz M, Tyras M, Stepinski J, Jankowska-Anyszka M, Bojarska E, Darzynkiewicz E.
RNA 2018 May; 24(5) 633-642
Grzela R, Nasilowska K, Lukaszewicz M, Tyras M, Stepinski J, Jankowska-Anyszka M, Bojarska E, Darzynkiewicz E.
RNA 2018 May; 24(5) 633-642
Modified ARCA analogs providing enhanced translational properties of capped mRNAs
Kocmik, I., Piecyk, K., Rudzinska, M., Niedzwiecka, A., Darzynkiewicz, E., Grzela, R., & Jankowska-Anyszka, M. (2018)
Cell Cycle
Kocmik, I., Piecyk, K., Rudzinska, M., Niedzwiecka, A., Darzynkiewicz, E., Grzela, R., & Jankowska-Anyszka, M. (2018)
Cell Cycle
Hydrolytic activity of human Nudt16 enzyme towards dinucleotide cap analogs and short capped oligonucleotides
Grzela, R., Nasilowska, K., Lukaszewicz, M., Tyras, M., Stepinski, J., Jankowska-Anyszka, M., ... & Darzynkiewicz, E. (2018)
RNA, rna-065698
Grzela, R., Nasilowska, K., Lukaszewicz, M., Tyras, M., Stepinski, J., Jankowska-Anyszka, M., ... & Darzynkiewicz, E. (2018)
RNA, rna-065698
Amino-Functionalized 5′ Cap Analogs as Tools for Site-Specific Sequence-Independent Labeling of mRNA.
Warminski, M., Sikorski, P. J., Warminska, Z., Lukaszewicz, M., Kropiwnicka, A., Zuberek, J., Darzynkiewicz, E., Kowalska, J. Darzynkiewicz, E., Kowalska, J. & Jemielity, J. (2017).
ioconjugate chemistry, 28(7), pp.1978-1992.
Warminski, M., Sikorski, P. J., Warminska, Z., Lukaszewicz, M., Kropiwnicka, A., Zuberek, J., Darzynkiewicz, E., Kowalska, J. Darzynkiewicz, E., Kowalska, J. & Jemielity, J. (2017).
ioconjugate chemistry, 28(7), pp.1978-1992.
The C-terminal residue of phage Vp16 PDF, the smallest peptide deformylase, acts as an offset element locking the active conformation.
Grzela, R., Nusbaum, J., Fieulaine, S., Lavecchia, F., Bienvenut, W. V., Dian, C., ... & Giglione, C. (2017).
Scientific reports, 7(1), 11041.
Grzela, R., Nusbaum, J., Fieulaine, S., Lavecchia, F., Bienvenut, W. V., Dian, C., ... & Giglione, C. (2017).
Scientific reports, 7(1), 11041.
Quantum dots use both LUMO and surface trap electrons in photoreduction proces, Journal of Luminescence
Darżynkiewicz, Z. M., Pędziwiatr, M., & Grzyb, J. (2017).
Journal of Luminescence, 183, 401-409.
Darżynkiewicz, Z. M., Pędziwiatr, M., & Grzyb, J. (2017).
Journal of Luminescence, 183, 401-409.
Molecular recognition of mRNA 5′ cap by 3′ poly(A)-specific ribonuclease (PARN) differs from interactions known for other cap-binding proteins
Niedzwiecka, A., Nilsson, P., Worch, R., Stepinski, J., Darzynkiewicz, E., & Virtanen, A. (2016)
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1864(4), 331-345
Niedzwiecka, A., Nilsson, P., Worch, R., Stepinski, J., Darzynkiewicz, E., & Virtanen, A. (2016)
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1864(4), 331-345
Phosphate-modified analogues of m7GTP and m7Gppppm7G – synthesis and biochemical properties
Ziemniak, M., Kowalska, J., Lukaszewicz, M., Zuberek, J., Wnek, K., Darzynkiewicz, E., & Jemielity, J. (2015)
Bioorganic & medicinal chemistry, 23(17), 5369-5381
Ziemniak, M., Kowalska, J., Lukaszewicz, M., Zuberek, J., Wnek, K., Darzynkiewicz, E., & Jemielity, J. (2015)
Bioorganic & medicinal chemistry, 23(17), 5369-5381
How to find the optimal partner-studies of snurportin 1 interactions with U snRNA 5’ TMG-cap analogues containing modified 2-amino group of 7-methylguanosine
Piecyk, K., Niedzwiecka, A., Ferenc-Mrozek, A., Lukaszewicz, M., Darzynkiewicz, E., & Jankowska-Anyszka, M. (2015)
Bioorganic & medicinal chemistry, 23(15), 4660-4668
Piecyk, K., Niedzwiecka, A., Ferenc-Mrozek, A., Lukaszewicz, M., Darzynkiewicz, E., & Jankowska-Anyszka, M. (2015)
Bioorganic & medicinal chemistry, 23(15), 4660-4668
Effect of different N7 substitution of dimucleotide cap analogs on the hydrolytic susceptibility towards scavenger decapping enzymes (DcpS)
Piecyk, K., Darzynkiewicz, Z. M., Jankowska-Anyszka, M., Ferenc-Mrozek, A., Stepinski, J., Darzynkiewicz, E., & Bojarska, E. (2015)
Biochemical and biophysical research communications, 464(1), 89-93
Piecyk, K., Darzynkiewicz, Z. M., Jankowska-Anyszka, M., Ferenc-Mrozek, A., Stepinski, J., Darzynkiewicz, E., & Bojarska, E. (2015)
Biochemical and biophysical research communications, 464(1), 89-93
District Features of Cap Binding by eIF4E1b Proteins
Kubacka, D., Miguel, R. N., Minshall, N., Darzynkiewicz, E., Standart, N., & Zuberek, J.
Journal of molecular biology, 427(2), 387-405.
Kubacka, D., Miguel, R. N., Minshall, N., Darzynkiewicz, E., Standart, N., & Zuberek, J.
Journal of molecular biology, 427(2), 387-405.
Five eIF4E isoforms from Arabidopsis thaliana are characterized by distinct features of cap analogs binding.
Kropiwnicka, A., Kuchta, K., Lukaszewicz, M., Kowalska, J., Jemielity, J., Ginalski, K., ... & Zuberek, J. (2015).
Biochemical and biophysical research communications, 456(1), 47-52.
Kropiwnicka, A., Kuchta, K., Lukaszewicz, M., Kowalska, J., Jemielity, J., Ginalski, K., ... & Zuberek, J. (2015).
Biochemical and biophysical research communications, 456(1), 47-52.
Distinct Features of Cap Binding by eIF4E1b Proteins
Kubacka, D., Miguel, R. N., Minshall, N., Darzynkiewicz, E., Standart, N., & Zuberek, J. (2015)
Journal of molecular biology, 427(2), 387-405
Kubacka, D., Miguel, R. N., Minshall, N., Darzynkiewicz, E., Standart, N., & Zuberek, J. (2015)
Journal of molecular biology, 427(2), 387-405
mRNA and snRNA Cap Analogs: Synthesis and Applications.
Stepinski, J., & Darzynkiewicz, E. (2014).
Chemical Biology of Nucleic Acids (pp. 511-561). Springer, Berlin, Heidelberg.
Stepinski, J., & Darzynkiewicz, E. (2014).
Chemical Biology of Nucleic Acids (pp. 511-561). Springer, Berlin, Heidelberg.
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei.
Freire, E. R., Vashisht, A. A., Malvezzi, A. M., Zuberek, J., Langousis, G., Saada, E. A., ... & Neto, O. P. D. M. (2014).
RNA, 20(8), 1272-1286.
Freire, E. R., Vashisht, A. A., Malvezzi, A. M., Zuberek, J., Langousis, G., Saada, E. A., ... & Neto, O. P. D. M. (2014).
RNA, 20(8), 1272-1286.
Triazole-containing monophosphate mRNA cap analogs as effective translation inhibitors.
Piecyk, K., Lukaszewicz, M., Darzynkiewicz, E., & Jankowska-Anyszka, M. (2014).
RNA, 20(10), 1539-1547.
Piecyk, K., Lukaszewicz, M., Darzynkiewicz, E., & Jankowska-Anyszka, M. (2014).
RNA, 20(10), 1539-1547.
Trypanosoma brucei translation initiation factor homolog EIF4E6 forms a tripartite cytosolic complex with EIF4G5 and a capping enzyme homolog.
Freire, E. R., Malvezzi, A. M., Vashisht, A. A., Zuberek, J., Saada, E. A., Langousis, G., ... & de Melo Neto, O. P. (2014).
Eukaryotic cell, 13(7), 896-908.
Freire, E. R., Malvezzi, A. M., Vashisht, A. A., Zuberek, J., Saada, E. A., Langousis, G., ... & de Melo Neto, O. P. (2014).
Eukaryotic cell, 13(7), 896-908.
Towards novel efficient and stable nuclear import signals: synthesis and properties of trimethylguanosine cap analogs modified within the 5′, 5′-triphosphate bridge.
Zytek, M., Kowalska, J., Lukaszewicz, M., Wojtczak, B. A., Zuberek, J., Ferenc-Mrozek, A., ... & Jemielity, J. (2014).
Organic & biomolecular chemistry, 12(45), 9184-9199.
Zytek, M., Kowalska, J., Lukaszewicz, M., Wojtczak, B. A., Zuberek, J., Ferenc-Mrozek, A., ... & Jemielity, J. (2014).
Organic & biomolecular chemistry, 12(45), 9184-9199.
Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes.
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014).
Nucleic acids research, 42(16), 10245-10264.
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014).
Nucleic acids research, 42(16), 10245-10264.
Structural analysis of human 2′-O-ribose methyltransferases involved in mRNA cap structure formation.
Smietanski, M., Werner, M., Purta, E., Kaminska, K. H., Stepinski, J., Darzynkiewicz, E., ... & Bujnicki, J. M. (2014).
Nature communications, 5, 3004.
Smietanski, M., Werner, M., Purta, E., Kaminska, K. H., Stepinski, J., Darzynkiewicz, E., ... & Bujnicki, J. M. (2014).
Nature communications, 5, 3004.
Synthesis, properities and biological activity of boranophosphate analogs of the mRNA cap: versalite tools for manipulation of therapeutically relevant cap-dependent processes
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014)
Nucleic acids research, 42(16), 10245-10264
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014)
Nucleic acids research, 42(16), 10245-10264
Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes.
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014).
Nucleic acids research, 42(16), 10245-10264.
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014).
Nucleic acids research, 42(16), 10245-10264.
Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes.
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014).
Nucleic acids research, 42(16), 10245-10264.
Kowalska, J., Wypijewska del Nogal, A., Darzynkiewicz, Z. M., Buck, J., Nicola, C., Kuhn, A. N., ... & Maciejczyk, M. (2014).
Nucleic acids research, 42(16), 10245-10264.
Trypanosoma brucei translation initiation factor homolog EIF4E6 forms a tripartite cytosolic complex with EIF4G5 and a capping enzyme homolog.
Freire, E. R., Malvezzi, A. M., Vashisht, A. A., Zuberek, J., Saada, E. A., Langousis, G., ... & de Melo Neto, O. P. (2014).
Eukaryotic cell, 13(7), 896-908.
Freire, E. R., Malvezzi, A. M., Vashisht, A. A., Zuberek, J., Saada, E. A., Langousis, G., ... & de Melo Neto, O. P. (2014).
Eukaryotic cell, 13(7), 896-908.
Synthesis and evaluation of stability of m 3 G-CAP analogues in serum-supplemented medium and cytosolic extract.
Honcharenko, M., Zytek, M., Bestas, B., Moreno, P., Jemielity, J., Darzynkiewicz, E., ... & Strömberg, R. (2013).
Bioorganic & medicinal chemistry, 21(24), 7921-7928.
Honcharenko, M., Zytek, M., Bestas, B., Moreno, P., Jemielity, J., Darzynkiewicz, E., ... & Strömberg, R. (2013).
Bioorganic & medicinal chemistry, 21(24), 7921-7928.
Synthesis and evaluation of fluorescent cap analogues for mRNA labelling.
Ziemniak, M., Szabelski, M., Lukaszewicz, M., Nowicka, A., Darzynkiewicz, E., Rhoads, R. E., ... & Jemielity, J. (2013).
RSC advances, 3(43), 20943-20958.
Ziemniak, M., Szabelski, M., Lukaszewicz, M., Nowicka, A., Darzynkiewicz, E., Rhoads, R. E., ... & Jemielity, J. (2013).
RSC advances, 3(43), 20943-20958.
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