| Position in the project: | Ph.D. Student |
| Scientific discipline: | Molecular and Cell Biology of Stem Cells |
| Job type (employment contract/stipend): | stipend |
| Number of job offers: | 2 |
| Remuneration/stipend amount/month: | 4 000 PLN / month |
| Position starts on: | Between 1st of August 2018, but not later than 1st of October 2018 |
| Maximum period of contract/stipend agreement: |
36 months |
| Institution: | Centre of New Technologies, University of Warsaw |
| Project leader: | Krzysztof Kobielak M.D., Ph.D. |
| Project title: | Reciprocal interaction between hair follicle Stem Cells and surrounding niche during skin and hair regeneration
Project is carried out within the TEAM programme of the Foundation for Polish Science |
| Project description: | The main goal of the project is to investigate the behavior of hair follicle stem cells (hfSCs) during stem cells (SCs) quiescence and activation. Currently, my laboratory shed light on how a competitive balance of BMP/WNT signaling regulate hfSCs but there is a gap in our scientific knowledge regarding how BMP/WNT signaling integrate the regulation of different molecular networks in hfSCs during hair cycle. Our long term objective is to understand how this regenerative hair cycling behavior is regulated at the molecular level, and how reciprocal interaction between hfSCs and surrounding niche changes during hair homeostasis and regeneration. |
| Key responsibilities include: | 1. A Ph.D. student will work with genetically modified mice (to obtain a required genetic background with mice crossing, inducing gene activation or ablation in vivo, mice genotyping, and analyzing of mouse phenotypes. 2. A Ph.D. student will be involved in cells isolation from varies tissues predominantly from skin of genetically modified mice. 3. Sorting of stem cells by FACS and preparation of samples for RNA–seq or Chip-seq analysis as well as stem cells culture in vitro 4. Tissue analysis by immunofluorescent /immunoisochemistry staining and /or in situ hybridization with use of microscope techniques |
| Profile of candidates/requirements: | 1. M.Sc. in Biology, Biotechnology or M.D. in Medicine 2. Good knowledge of English, 3. Knowledge of Adobe Photoshop, Adobe Illustrator, PowerPoint 4.Team work skills 5. Experience or knowledge in laboratory work (gel electrophoresis, PCR, RT-PCR, q-PCR, DNA/RNA/Protein extraction/purification, DNA cloning, western blot, cryo- and paraffin- sectionings, cell culture, FACS sorting, immunofluorescent /immunoisochemistry staining, in situ hybridization, mouse breeding/numbering/genotyping, some basic mouse surgeries, microscope techniques). |
| Required documents: | 1. Curriculum Vitae (CV) 2. Cover letter, describing Candidate motivation 3. M.Sc. or M.D. certificate 4. One or more letters of recommendation from a scientist who is familiar with the Candidate (submitted directly to email address below) 5. Information on scientific publications, scholarships, prizes and awards or other relevant documents demonstrating the excellence of Candidate 6. A list of attended conferences with titles and authors of presentations 7. A personal data processing agreement |
| We offer: | Monthly scholarship approximately 4 000 PLN. The initial appointment is one-year with possibility of renewal up to 36 months. The appointment should start between 1st of August 2018, but not later than 1st of October 2018. |
| Please submit the following documents to: | k.kobielak@dev.dev.cent.uw.edu.pl/en (entitle your email “Team Young doctor”). |
| Application deadline: | 30th June 2018 |
| For more details about the position please visit (website/webpage address): | http://www.dev.dev.cent.uw.edu.pl/en |
| Euraxess job/stipend offer (in case of PhD and postdoc positions): | https://www.euraxess.pl/jobs |
